ABSTRACT
Objective To observe the inhibitory effects of Yiqi Jiedu Recipe(YJR)on transplanted Lewis lung cancer in mice and to explore its relative mechanism. Methods In-vivo mouse model of transplanted Lewis lung cancer was established. Twenty modeled mice were divided into model group,low- and high-dose YJR groups, and cisplatin group, 5 mice in each group. After treatment, the mouse general situations, body mass, body mass excluding tumor mass,tumor mass,tumor index,tumor volume,and tumor-inhibition rate were detected. In the in-vitro experiments, the inhibitory effect of YJR on the proliferation of A549 and H1299 cells was evaluated by CCK8 assays, and the effect of YJR on the apoptosis of A549 and H1299 cells was evaluated by flow cytometry assay. Bioinformatics analysis was carried out to identify the critical function,pathways and genes with bioinformatics mining method. Results Compared with the model group, high-dose YJR group and cisplatin group significantly inhibited the tumor growth of the transplanted Lewis lung cancer in mice(P<0.05 or P < 0.001). The experimental results in vitro showed that YJR significantly inhibited lung cell growth, and induced lung cell apoptosis. Bioinformatics analysis results showed that the inhibitory effects of YJR was probably related to multiple paths including apoptosis,cell cycle,autophagy,etc.,and promoting apoptosis may be the important way. YJR played a role in promoting apoptosis via AKT, HSP90, BCL2, CASP9 and activation of PI3K-AKT pathway. Conclusion YJR exhibits anti-tumor actions through regulating the targets AKT,HSP90, BCL2, CASP9 and affecting PI3K-AKT pathway to promote apoptosis and inhibit cell proliferation, thus to suppress the growth of transplanted tumor.